Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition,is
a must-have collection of methods and procedures that create a single,
continuous, comprehensive project that teaches students basic molecular
techniques. It is an indispensable tool for introducing advanced
undergraduates and beginning graduate students to the techniques of
recombinant DNA technology, or gene cloning and expression. The
techniques used in basic research and biotechnology laboratories are
covered in detail. Students gain hands-on experience from start to
finish in subcloning a gene into an expression vector, through
purification of the recombinant protein.
The fourth edition is
updated with new laboratory exercises, designed for a typical 15-week
semester, rather than a 4-week intensive course. The "project" approach
to experiments has been student and instructor tested for years:
students still follow a cloning project through to completion,
culminating in the purification of recombinant protein. It takes
advantage of the enhanced green fluorescent protein - students can
actually visualize positive clones following IPTG induction.
Student-tested labs proven successful in real classroom laboratories
Testbank included on companion website for additional testing and practice
Exercises simulate a cloning project that would be performed in a real research lab
"Project" approach to experiments gives students an overview of the entire process
Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
By: Susan Carson, Heather Miller, Melissa C. Srougi, D. Scott Witherow
ID: SC - 1462
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