Showing posts with label Biotechnology. Show all posts
Showing posts with label Biotechnology. Show all posts

* U OBRADI - Synthetic DNA: Methods and Protocols by Randall Hughe

This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology.

Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

* U OBRADI - Artificial Cells: Biotechnology, Nanomedicine, Regenerative Medicine, Blood Substitutes, Bioencapsulation, and Cell/Stem Cell Therapy by Thomas Ming Swi Chang

This is the first book that provides a comprehensive review of the entire area of artificial cells. The author, a pioneer of the field, invented the first artificial cells some 50 years ago and has continued to carry out active research in this field.

Since then, there have been explosive research activities around the world on artificial cells, especially in fields related to biotechnology, nanomedicine, cell therapy, blood substitutes, drug delivery and others. However, instead of the term “artificial cells,” many authors use other terminologies such as blood substitutes, bioencapsulation, liposomes, nanoparticles and so on.

Furthermore, papers in this highly interdisciplinary area are published in numerous journals specializing in chemistry, medicine, surgery, bioengineering and others, while books in this area are mostly multi-authored, describing very specific and narrow areas. As a result, any meaningful literature search for a complete idea of the present status of the whole field of artificial cells is impossible.

This monograph is written to fill this gap by including all those areas in artificial cells that are disguised under different terminologies. Each chapter begins with a detailed overview, followed by detailed examples of the author's own research and a full description of his methods and procedures.

Readers interested in a detailed overview of the whole area can read from cover to cover, omitting the methods section at the end of each chapter; while those entering this area of research will find the detailed methods and procedures very useful.

SCIL - 150/1 | Vaccine Design: Methods and Protocols, Volume 1: Vaccines for Human Diseases by Sunil Thomas

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals.

Volume 1: Vaccines for Human Diseases has an introductory section on how vaccines impacted diseases, the immunological mechanism of vaccines, future challenges for vaccinologists, and current trends in vaccinology.
The design of human vaccines for viral, bacterial, fungal, parasitic and prion diseases as well as vaccines for drug abuse, allergy, and tumor vaccines are also described in this volume.
As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 1: Vaccines for Human Diseases aims to ensure successful results in the further study of this vital field.

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SCIL - 150/2 | Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases by Sunil Thomas

This text provides a practical guide providing step-by-step protocol to design and develop vaccines.
Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals.

Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property.

Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field.

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SCIL - 149/1 | Biosensors and Biodetection: Methods and Protocols Volume 1: Optical-Based Detectors

This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities.

Chapters in Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition chapters focus on direct and indirect optical detectors including surface plasmon resonance, interferometric sensors, charge-coupled device (CCD) based detectors, spectrometry, and many other cutting-edge optical biosensors technologies.

Written in the format of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.

Authoritative and practical, Biosensors and Biodetection: Methods and Protocols, Volume 1: Optical-Based Detectors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail.

The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.

By: Avraham Rasooly, Ben Prickril (eds.)

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SCIL - 149/2 | Biosensors and Biodetection: Methods and Protocols, Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors

This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities.

Chapters in Biosensors and Biodetection: Methods and Protocols Volume 2, Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition focus on electrochemical biosensors including amperometric, impedance and voltammetric sensors, bioelectronic, piezoelectric, cellular, and molecular biosensors.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.

Authoritative and practical, Biosensors and Biodetection: Methods and Protocols Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail.

The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.

By: Ben Prickril, Avraham Rasooly (eds.)

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SC - 1646 | Quantitative Real-Time PCR Methods and Protocols by Roberto Biassoni, Alessandro Raso

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications.

With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

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SC - 1645 | PCR: Methods and Protocols by Lucília Domingues

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.

Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

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SC - 1629 | Hidden Treasures in Contemporary RNA Sequencing

Advances in RNA-sequencing (RNA-seq) technologies have provided an unprecedented opportunity to explore the gene expression landscape across individuals, tissues, and environments by efficiently profiling the RNA sequences present in the samples.

When a reference genome sequence or a transcriptome of the sample is available, mapping-based RNA-seq analysis protocols align the RNA-seq reads to the reference sequences, identify novel transcripts, and quantify the abundance of expressed transcripts.The reads that fail to map to the human reference, known as unmapped reads, are a large and often overlooked output of standard RNA-seq analyses.

Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled.
Reads can also remain unmapped due to unknown transcripts, recombined B and T cell receptor sequences, A-to-G mismatches from A-to-I RNA editing, trans-splicing, gene fusion, circular RNAs, and the presence of non-host RNA sequences (e.g. bacterial, fungal, and viral organisms).
Unmapped reads represent a rich resource for the study of B and T cell receptor repertoires and the human microbiome system—without incurring the expense of additional targeted sequencing.

This book introduces and describes the Read Origin Protocol (ROP), a tool that identifies the origin of both mapped and unmapped reads.

The protocol first identifies human reads using a standard high-throughput algorithm to map them onto a reference genome and transcriptome. After alignment, reads are grouped into genomic (e.g. CDS, UTRs, introns) and repetitive (e.g. SINEs, LINEs, LTRs) categories. The rest of the ROP protocol characterizes the remaining unmapped reads, which failed to map to the human reference sequences.

By: Serghei Mangul, Harry Taegyun Yang, Eleazar Eskin, Noah Zaitlen

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SCIL - 147 | Basics of Cell Biology and Biotechnology by Arvinder Singh, Anil Gupta

This is an endeavor to make the graduate students acquainted with the basic knowledge of biotechnology.

The book has been written to meet the requirements of B.tech 1st year students (all branches) studying in various engineering colleges affiliated to K.U. Kurukshetra and M.D.U, Rohtak. The book also covers some part of the syllabus of B.Sc. course, and will be of immense help for degree college students.

It has been designed to disseminate key information on various aspects of cell biology, genetics, recombinant DNA technology, application of biotechnology in various fields and role of biology in allied fields.

The book is divided into 4 units containing 8 chapters in total; each chapter is ended with some selected questions.

Dr. Arvinder Singh:
Dr. Arvinder Singh is presently working as Assistant Professor of Botany in Department of Botany, Akal University, Talwandi Sabo, Bathinda. Before joining this University, he had taught the basics of biotechnology to B.Tech. students at KITM, Kurukshetra for five years. 

Dr. Anil Gupta : 

Dr. Anil Gupta is Associate Professor of Botany at University College in Kurukshetra University. He has a vast experience of 18 years of teaching graduate classes.

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SCIL - 142 | New and Future Developments in Microbial Biotechnology and Bioengineering From Cellulose to Cellulase Strategies to Improve Biofuel Production

New and Future Developments in Microbial Biotechnology and Bioengineering: From Cellulose to Cellulase: Strategies to Improve Biofuel Production outlines new methods for the industrial production of the cellulose enzyme.

The book compares the various processes for the production of biofuels, including the cost of cellulose production and availability. Biofuels are considered to be the main alternatives to fossil fuels in reducing environmental pollution and climate change. Currently, all existing biofuel production is suffering because of the high costs of production processes. As a result, cost effective practical implementation is needed to make this a viable energy alternative.

By: Neha Srivastava (Editor), Manish Srivastava (Editor), P.K. Mishra (Editor), P.W. Ramteke (Editor), Ram Lakhan Singh (Editor)

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SCIL - 141 | New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Secondary Metabolites Biochemistry and Applications

New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Secondary Metabolites Biochemistry and Applications examines the areas of biotechnology and chemical engineering, covering aspects of plants, bacteria and machines, and using microbes as factories.

The book is aimed at undergraduates, post-graduates and researchers studying microbial secondary metabolites, and is an invaluable reference source for biochemical engineers working in biotechnology, manipulating microbes, and developing new uses for bacteria and fungi.
The applications of secondary metabolites in biotechnology, pharmaceuticals, diagnostics and medical device development are also extensively covered.

The book integrates the aforementioned frontline branches into an interdisciplinary research work to satisfy those working in biotechnology, chemical engineering, alternative fuel development, diagnostics and pharmaceuticals. Chapters related to important research work on applications of microbial secondary metabolites are written by specialists in the various disciplines from the international community.

- Compiles the latest developments in the area of microbial secondary metabolites
- Authored by the top international researchers in this area
- Includes information related to nearly all areas of a microbial secondary metabolites system

By: Vijai G. Gupta (editor), Anita Pandey (editor)

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SCIL - 140 | New and Future Developments in Microbial Biotechnology and Bioengineering. Crop Improvement through Microbial Biotechnology

Crop Improvement through Microbial Biotechnology explains how certain techniques can be used to manipulate plant growth and development, focusing on the cross-kingdom transfer of genes to incorporate novel phenotypes in plants, including the utilization of microbes at every step, from cloning and characterization, to the production of a genetically engineered plant.

This book covers microbial biotechnology in sustainable agriculture, aiming to improve crop productivity under stress conditions. It includes sections on genes encoding avirulence factors of bacteria and fungi, viral coat proteins of plant viruses, chitinase from fungi, virulence factors from nematodes and mycoplasma, insecticidal toxins from Bacillus thuringiensis, and herbicide tolerance enzymes from bacteria.

- Introduces the principles of microbial biotechnology and its application in crop improvement
- Lists various new developments in enhancing plant productivity and efficiency
- Explains the mechanisms of plant/microbial interactions and the beneficial use of these interactions in crop improvement
- Explores various bacteria classes and their beneficial effects in plant growth and efficiency

By: Ram Prasad, Sarvajeet Singh Gill, Narendra Tuteja

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SCIL - 139 | New and Future Developments in Microbial Biotechnology and Bioengineering. Microbial Cellulase System Properties and Applications

New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Cellulase System Properties and Applications covers the biochemistry of cellulase system, its mechanisms of action, and its industrial applications. Research has shed new light on the mechanisms of microbial cellulase production and has led to the development of technologies for production and applications of cellulose degrading enzymes.

The biological aspects of processing of cellulosic biomass have become the crux of future research involving cellulases and cellulolytic microorganisms, as they are being commercially produced by several industries globally and are widely being used in food, animal feed, fermentation, agriculture, pulp and paper, and textile applications. The book discusses modern biotechnology tools, especially in the area of microbial genetics, novel enzymes, and new enzyme and the applications in various industries.

As a professional reference, this new book is useful to all researchers working with microbial cellulase system, both academic institutions and industry-based research bodies, as well as to teachers, graduate, and postgraduate students with information on continuous developments in microbial cellulase system. The book provides an indispensable reference source for chemists, biochemical engineers/bioengineers, biochemists, biotechnologists and researchers who want to know about the unique properties of this microbe and explore its future applications.

- Compiles the latest developments made and currently undergoing in the area of microbial cellulase system
- Chapters are contributed from top researchers on this area around the globe
- Includes information related to almost all areas of microbial cellulase system
- Extensive cover of current industrial applications and discusses potential future applications

By: Vijai G. Gupta

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SCIL - 138 | New and Future Developments in Microbial Biotechnology and Bioengineering. Aspergillus System Properties and Applications

New and Future Developments in Microbial Biotechnology and Bioengineering: Aspergillus System Properties and Applications provides information on emerging issues related to recent advancements in aspergillus research and its applications in bioprocess technology, chemical engineering, genome biology, molecular taxonomy, secondary and metabolite production, industrial process and biofuels/bioenergy research, and alternative fuel development. 

The book covers the various novel enzymes secreted by these fungi and their specific use in the food, textile, pulp and paper, biocellulosic ethanol production, and other industries.

The book describes research and experimentation on aspergillus activity and directly connects them to their use in bioprocess technology, chemical engineering, bioremediation process, secondary metabolite production, pharmaceutical processes, protein production, industrial process, biofuels/bioenergy research, and alternative fuel development. 

Readers will find this book to be an indispensable resource for biotechnologists, biochemical engineers, biochemists, microbiologists, bioinformatics researchers, and other biologists who are interested in learning about the potential applications of these fungi.

- Compiles available, up-to-date information on recent developments made in the study of aspergillus system properties
- Contains global content from pioneering international authors
- Presents current research efforts and links them to various applications, including uses in foods, textiles, pulp and paper, and in biocellulosic ethanol production
- Provides an indispensable resource for biologists who are interested in learning about the potential applications of the fungi aspergillus

By: Vijai G. Gupta

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SCIL - 131/1 | Plant Biology and Biotechnology, Volume I: Plant Diversity, Organization, Function and Improvement

This volume offers a much-needed compilation of essential reviews on diverse aspects of plant biology, written by eminent botanists. These reviews effectively cover a wide range of aspects of plant biology that have contemporary relevance. At the same time they integrate classical morphology with molecular biology, physiology with pattern formation, growth with genomics, development with morphogenesis, and classical crop-improvement techniques with modern breeding methodologies. Classical botany has been transformed into cutting-edge plant biology, thus providing the theoretical basis for plant biotechnology.

It goes without saying that biotechnology has emerged as a powerful discipline of Biology in the last three decades. More than 90% of the total requirements for the biotechnology industry are contributed by plants and microbes, in terms of goods and services. There are however substantial plant and microbial resources that are waiting for biotechnological exploitation in the near future through effective bioprospection. In order to exploit plants and microbes for their useful products and processes, we need to first understand their basic structure, organization, growth and development, cellular process and overall biology. We also need to identify and develop strategies to improve the productivity of plants.

In view of the above, in this two-volume book on plant biology and biotechnology, the first volume is devoted to various aspects of plant biology and crop improvement. It includes 33 chapters contributed by 50 researchers, each of which is an expert in his/her own field of research. 

The book begins with an introductory chapter that gives a lucid account on the past, present and future of plant biology, thereby providing a perfect historical foundation for the chapters that follow. Four chapters are devoted to details on the structural and developmental aspects of the structures of plants and their principal organs. These chapters provide the molecular biological basis for the regulation of morphogenesis of the form of plants and their organs, involving control at the cellular and tissue levels. Details on biodiversity, the basic raw material for biotechnology, are discussed in a separate chapter, in which emphasis is placed on the genetic, species and ecosystem diversities and their conservation.

Since fungi and other microbes form an important component of the overall biodiversity, special attention is paid to the treatment of fungi and other microbes in this volume. 

Microbial endosymbionts associated with plants and phosphate solubilizing microbes in the rhizosphere of plants are exhaustively treated in two separate chapters. The reproductive strategies of bryophytes and an overview on Cycads form the subject matter of another two chapters, thus fulfilling the need to deal with the non-flowering Embryophyte group of plants.

Angiosperms, the most important group of plants from a biotechnological perspective, are examined exhaustively in this volume. The chapters on angiosperms provide an overview and cover the genetic basis of flowers development, pre-and post-fertilization reproductive growth and development, seed biology and technology, plant secondary metabolism, photosynthesis, and plant volatile chemicals. 


Microalgalnutra-pharmaceuticals, vegetable-oil-based nutraceuticals and the importance of alien crop resources and underutilized crops for food and nutritional security form the topics of three other chapters in this volume. There is also a special chapter on the applications of remote sensing in the plant sciences, which also provides information on biodiversity distribution.

The editors of this volume believe the wide range of basic topics on plant biology that have great relevance in biotechnology covered will be of great interest to students, researchers and teachers of botany and plant biotechnology alike.

By: Bir Bahadur (Editor), Manchikatla Venkat Rajam, Leela Sahijram, K.V. Krishnamur

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SCIL - 131/2 | Plant Biology and Biotechnology, Volume II: Plant Genomics and Biotechnology

Plant genomics and biotechnology have recently made enormous strides, and hold the potential to benefit agriculture, the environment and various other dimensions of the human endeavor. It is no exaggeration to claim that the twenty-first century belongs to biotechnology. Knowledge generation in this field is growing at a frenetic pace, and keeping abreast of the latest advances and calls on us to double our efforts.

Volume II of this two-part series addresses cutting-edge aspects of plant genomics and biotechnology. It includes 37 chapters contributed by over 70 researchers, each of which is an expert in his/her own field of research.

Biotechnology has helped to solve many conundrums of plant life that had long remained a mystery to mankind. This volume opens with an exhaustive chapter on the role played by thale cress, Arabidopsis thaliana, which is believed to be the Drosophila of the plant kingdom and an invaluable model plant for understanding basic concepts in plant biology. 

This is followed by chapters on bioremediation, biofuels and biofertilizers through microalgal manipulation, making it a commercializable prospect; discerning finer details of biotic stress with plant-fungal interactions; and the dynamics of abiotic and biotic stresses, which also figure elsewhere in the book.

The significance of molecular markers, marker assisted selection and techniques are covered in a dedicated chapter, as are comprehensive reviews on plant molecular biology, DNA fingerprinting techniques, genomic structure and functional genomics. 

A chapter dedicated to organellar genomes provides extensive information on this important aspect. Elsewhere in the book, the newly emerging area of epigenetics is presented as seen through the lens of biotechnology, showcasing the pivotal role of DNA methylation in effecting permanent and transient changes to the genome. Exclusive chapters deal with bioinformatics and systems biology. Handy tools for practical applications such as somatic embryogenesis and micropropagation are included to provide frontline information to entrepreneurs, as is a chapter on somaclonal variation.

Overcoming barriers to sexual incompatibility has also long been a focus of biotechnology, and is addressed in chapters on wide hybridization and hybrid embryo rescue. Another area of accomplishing triploids through endosperm culture is included as a non-conventional breeding strategy. Secondary metabolite production through tissue cultures, which is of importance to industrial scientists, is also covered.

Worldwide exchange of plant genetic material is currently an essential topic, as is conserving natural resources in situ. Chapters on in vitro conservation of extant, threatened and other valuable germplasms, gene banking and related issues are included, along with an extensive account of the biotechnology of spices – the low-volume, high-value crops.

Metabolic engineering is another emerging field that provides commercial opportunities. As is well known, there is widespread concern over genetically modified crops among the public. GM crops are covered, as are genetic engineering strategies for combating biotic and abiotic stresses where no other solutions are in sight. RNAi- and micro RNA- based strategies for crop improvement have proved to offer novel alternatives to the existing non-conventional techniques, and detailed information on these aspects is also included. The book’s last five chapters are devoted to presenting the various aspects of environmental, marine, desert and rural biotechnology.

The state-of-the-art coverage on a wide range of plant genomics and biotechnology topics will be of great interest to post-graduate students and researchers, including the employees of seed and biotechnology companies, and to instructors in the fields of plant genetics, breeding and biotechnology.

By: Bir Bahadur (Editor), Manchikatla Venkat Rajam (Editor), Leela Sahijram (Editor), K.V. Krishnamurthy (Editor)

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SC - 1625 | Plant Biotechnology Principles and Applications

The book traces the roots of plant biotechnology from the basic sciences to current applications in the biological and agricultural sciences, industry, and medicine. Providing intriguing opportunities to manipulate plant genetic and metabolic systems, plant biotechnology has now become an exciting area of research.

The book vividly describes the processes and methods used to genetically engineer plants for agricultural, environmental and industrial purposes, while also discussing related bioethical and biosafety issues. It also highlights important factors that are often overlooked by methodologies used to develop plants' tolerance against biotic and abiotic stresses and in the development of special foods, bio-chemicals, and pharmaceuticals.

The topics discussed will be of considerable interest to both graduate and postgraduate students. Further, the book offers an ideal reference guide for teachers and researcher alike, bridging the gap between fundamental and advanced approaches.

By: Malik Zainul Abdin (Editor), Usha Khantwal (Editor), M. Kamaluddin (Editor), Athar Ali (Editor)

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SC - 1624 | Modern Industrial Microbiology and Biotechnology by Nduka Okafor, Benedict C. Okeke

The field of industrial microbiology involves a thorough knowledge of the microbial physiology behind the processes in the large-scale, profit-oriented production of microbe-related goods which are the subject of the field.

In recent times a paradigm shift has occurred, and a molecular understanding of the various processes by which plants, animals and microorganisms are manipulated is now central to industrial microbiology. Thus the various applications of industrial microbiology are covered broadly, with emphasis on the physiological and genomic principles behind these applications. 

Relevance of the new elements such as bioinformatics, genomics, proteomics, site-directed mutation and metabolic engineering, which have necessitated the paradigm shift in industrial microbiology are discussed.

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SC - 1623 | The ABCs of Gene Cloning by Dominic W. S. Wong

Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner.

  - Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning.
  - Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis.
  - Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas.

The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.

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